Our lab is interested in human immunology at the single-cell and single-molecule level, with foci in:

1. T cell and car-t cell signaling

2. car-t cell engineering

3. Nanoparticle-based immunotherapies

selected techniques

Single-Cell Sequencing

Immune cells are highly heterogeneous. In order to examine individual immune cells in different physiological and pathological conditions, our lab is experienced in single-cell workflows. We also use other bulk, next-gen library preparations and analyses.

Lipid Nanoparticles

Our lab has experience with clinically-used LNP formulations as well as our own designs. LNPs are used in our lab for a variety of purposes, including vaccination and gene transfer.

Single-Molecule & Super-Resolution Microscopy

Our lab applies single-molecule and super-resolution imaging techniques to investigate the dynamics, interactions, and signaling of molecules on live immune cell surfaces.

CAR Engineering and genetic manipulation

Our lab is experienced in cloning, viral production, and genetic manipulation of primary human cells and cell lines. Examples include expression of CARs, IVTT, gene knockdown via shRNA, and CRISPR.