Single-Cell Sequencing

Immune cells are highly heterogeneous. We will use next generation sequencing technologies to examine individual immune cells in different physiological and pathological conditions, thereby providing a systemic understanding of the immune function of single cells in the context of its microenvironment.

NGS_MainFigure_CristinaSpanò-696x508.jpg

Single Molecule Kinetics and Force Analyses

Single-cell micropipette adhesion assay measures in situ molecular interactions at the single-cell and single-molecule level, biomembrane force probe (BFP) is an advanced version of micropipette with a much higher spatiotemporal resolution of ~3nm and ~1 ms, and molecular dynamic (MD) simulation is a computer based simulation to reveal protein structural changes and dynamics at the single-atomic level. We use micropipette adhesion assay, BFP, and MD simulation to study single immune molecules in vitro and in silico.

A-Phase-contrast-images-of-wildtype-cells-in-a-micro-pipette-aspiration-experiment-as.png

Single Molecule and Super-Resolution Microscopy

We apply single-molecule imaging technique to investigate the dynamics, interactions, and signaling of immune molecules on live cell surfaces. We also use super-resolution microscopy to map the nanostructures (~25 nm resolution) of cell surface molecules.

static1.squarespace.com.jpeg

Single-Cell Mass Cytometry

Single-cell mass cytometry is an advanced version of flow cytometry without the issues of spectral overlap. It uses heavy metal isotopes to label molecules of interst and then labeled cells are analyzed by high-throughput mass spectrometry. Single-cell mass cytometry can simultaneously measure >40 proteins of a single cell.

1-s2.0-S0958166914001323-gr1.jpg